Full product ion spectra including accurate masses substantiate compound identification by matching their masses and ratios with authentic standards. For most of the analytes coefficient of variation were 10% or lower and LOQs were determined significantly below 1ng/ml. Food and Drug Administration (FDA) and the European Medicines Agency (EMA) guidelines. It has a run-time of 5.3min and was validated according to the U.S. androstenedione, corticosterone, cortisol, cortisone, 11-deoxycortisol, 17-hydroxyprogesterone, progesterone, and testosterone. The LC-MS/HR-MS method using a quadrupole-Orbitrap analyzer includes eight steroid hormones i.e. Samples were prepared by automated liquid-liquid extraction with MTBE. Separation of isobaric interferences reduces background noise and avoids overestimation. Application of HR-MS demonstrates an enhanced selectivity compared to low mass resolution. Here we introduce liquid chromatography-high resolution tandem mass spectrometry (LC-MS/HR-MS) to further increase selectivity of steroid hormone quantification. To improve selectivity mass spectrometric analysis of steroid hormones by liquid chromatography-tandem mass spectrometry (LC-MS/MS) has been introduced in the clinical laboratory over the past years usually with low mass resolution triple-quadrupole instruments or more recently by high resolution mass spectrometry (HR-MS). Quantification of steroid hormones in human serum by liquid chromatography-high resolution tandem mass spectrometry.Ī limited specificity is inherent to immunoassays for steroid hormone analysis. The prospect of LC-HRMS in forensic toxicology analysis is also included. This review focuses on the applications of LC-HRMS in screening and identification of drug-of-abuse, prescription drugs, pesticide and stimulant. Depending on its powerful separation ability, superhigh resolution and accurate mass measurement, combined with the two levels spectrum database matching and abundance ratio of isotope ion, the liquid chromatography-high resolution mass spectrometry (LC-HRMS) analyzers have increasingly advantage in screening and identification of chemical compound. Li, Xiao-Wen Shen, Bao-Hua Zhuo, Xian-Yiĭue to the diversity of toxicologically relevant substances, the uncertainty of target compounds and the specificity of samples, toxicological screening techniques have always been valued by the forensic toxicologists.
The most interesting quantitative applications, where a significant contribution of gas chromatography with high- resolution mass spectrometry over the currently used methods is expected, will be discussed as well.
The main attention is paid to various approaches and applications of gas chromatography coupled to high- resolution mass spectrometry for non-target screening to identify contaminants and to characterize the chemical composition of environmental, food, and biological samples. This review is focused on the development and the most interesting applications of gas chromatography coupled to high- resolution mass spectrometry towards analysis of environmental matrices, biological fluids, and food safety since 2010. The introduction of a quadrupole high- resolution time-of-flight mass analyzer broadened interest in this method and novel applications were developed, especially for multi-target screening purposes. Gas chromatography with high- resolution mass spectrometry quantitative analyses was previously focused on the determination of dioxins and related compounds using magnetic sector type analyzers, a standing requirement of many international standards. These features designate gas chromatography with high- resolution mass spectrometry as the first choice for identification and structure elucidation of unknown volatile and semi-volatile organic compounds. Gas chromatography coupled to high- resolution mass spectrometry is a powerful analytical method that combines excellent separation power of gas chromatography with improved identification based on an accurate mass measurement. Recent applications of gas chromatography with high- resolution mass spectrometry.
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